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The Influence of Surface Charge on the Antiviral Effect of Curcumin Loaded in Nanocarrier System

Author(s):

Najwa Nabila, Siti Riqqah Hassan, Gladys Pinkan Larasati, Benediktus Yohan, R. Tedjo Sasmono, Annis Catur Adi, Ferry Iskandar and Heni Rachmawati*   Pages 1 - 7 ( 7 )

Abstract:


Background : Curcumin is a well-documented bioactive compound present in Curcuma sp., a tropical medicinal plant. This substance exhibits broad spectrum biological activities including antivirus. Despite, the lack of pharmaceutical properties of curcumin limits its clinical use.

Objective : This study aims to produce curcumin nanoemulsion with different surface charge (curcumin (+) nanoemulsion and curcumin (-) nanoemulsion) and to evaluate its physical characteristics, in vitro cell cytotoxicity and antiviral activity against dengue virus (DENV) 1 and 2.

Methods : Two forms of nanoemulsion were prepared which were differed from their surface charge through spontaneous procedure resulting similar characteristics except the zeta potential value. Cytotoxicity was determined using RT-PCR method in A549 cell line and anti-DENV properties were determined by calculation of inhibitory concentration 50 (IC50) value.

Results : The positively charge of curcumin-loaded nanoemulsion showed better effect in reducing the viral replication represented by lower IC50 value. In addition, DENV-1 was more sensitive and responsive to curcumin as compared to DENV-2.

Conclusion : Positive surface charge of curcumin-loaded nanoemulsion improves the antiviral effect of the curcumin suggesting a promising approach for alternative treatment for dengue virus infection.

Keywords:

curcumin, dengue virus, nanoemulsion, surface charge, antiviral activity

Affiliation:

School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung, 40132, School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung, 40132, School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung, 40132, Eijkman Institute for Molecular Biology, Ministry of Research, Technology, and Higher Education, Jl. Diponegoro 69 Jakarta 10430, Eijkman Institute for Molecular Biology, Ministry of Research, Technology, and Higher Education, Jl. Diponegoro 69 Jakarta 10430, Department of Nutrition, Faculty of Public Health, University of Airlangga, Mulyorejo, Surabaya, 60115, Research Center for Nanosciences and Nanotechnology, Bandung Institute of Technology, Ganesa 10, Bandung, 40132, School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung, 40132



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