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Antiviral Action of Curcumin Encapsulated in Nanoemulsion against Four Serotypes of Dengue Virus

[ Vol. 8 , Issue. 1 ]

Author(s):

Najwa Nabila, Nadia Khansa Suada, Dionisius Denis, Benediktus Yohan, Annis Catur Adi, Anna Surgean Veterini, Atsarina Larasati Anindya, R. Tedjo Sasmono and Heni Rachmawati*   Pages 54 - 62 ( 9 )

Abstract:


Background: Curcumin has been used as a traditional medicine showing antiinflammatory, antimicrobial, and antiviral properties. Despite the promising potentials, curcumin-based drug development is hindered due to its poor solubility and cell uptake.

Objective: This study aims to produce curcumin nanoemulsion (nanocurcumin) and evaluate its physical characteristics and in vitro cell cytotoxicity and antiviral activity against dengue virus (DENV).

Methods: Nanocurcumin was generated by self-nanoemulsion technique. Cytotoxicity was determined using MTT assay in A549 cell line. Anti-DENV properties were determined by calculation of inhibitory concentration 50 (IC50) and plaque assay.

Results: The resulting nanoemulsion showed uniform droplet size distribution with the average droplet size of 40.85 ± 0.919 nm. Nanocurcumin exhibited higher cell cytotoxicity compared to curcumin solution and may be explained by better cell uptake. Nanocurcumin treatment suppressed DENV growth, although no significant difference observed compared to the curcumin solution counterpart. Greater virus reduction was observed for DENV-1 and DENV-2.

Conclusion: The synthesis of nanocurcumin improved curcumin physicochemical properties with potential as antiviral against DENV.

Keywords:

A549 cell line, antiviral activity, cellular uptake, Curcumin, dengue virus, nanoemulsion.

Affiliation:

School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung, 40132, School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung, 40132, Eijkman Institute for Molecular Biology, Ministry of Research, Technology, and Higher Education, Jl. Diponegoro 69, Jakarta 10430, Eijkman Institute for Molecular Biology, Ministry of Research, Technology, and Higher Education, Jl. Diponegoro 69, Jakarta 10430, Department of Nutrition, Faculty of Public Health, University of Airlangga, Kampus C Mulyorejo, Jl. Mulyosari, Surabaya, 60115, Department of Anesthesiology and Intensive Care, Dr. Soetomo General-Academic Hospital and Medical Faculty, University of Airlangga, Jl. Mayjen. Prof. Dr. Moestopo 47, Surabaya 60132, Research Center for Nanosciences and Nanotechnology, Bandung Institute of Technology, Ganesa 10, Bandung 40132, Eijkman Institute for Molecular Biology, Ministry of Research, Technology, and Higher Education, Jl. Diponegoro 69, Jakarta 10430, School of Pharmacy, Bandung Institute of Technology, Ganesa 10, Bandung, 40132

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